CARD10 antibody (C-Term)

Catalog No. ABIN6990395

The Rabbit Polyclonal anti-CARD10 antibody has been validated for WB, ELISA, IF and ICC. It is suitable to detect CARD10 in samples from Human and Mouse.

Quick Overview for CARD10 antibody (C-Term) (ABIN6990395)

Target: CARD10 (Caspase Recruitment Domain Family, Member 10 (CARD10))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CARD10 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-CARD10 Antibody

Binding Specificity: C-Term

Purification: CARD10 Antibody is immunoaffinity purified .

Immunogen: CARD10 antibody was raised against a synthetic peptide corresponding to 14 amino acids at the C-terminus of human CARD10. The immunogen is located within the last 50 amino acids of CARD10.

Isotype: IgG

Application Details

Application Notes: CARD10 antibody can be used for detection of CARD10 by Western blot at 5 μ,g/mL. Antibody can also be used for immunocytochemistry starting at 10 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in mouse samples, Immunocytochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: CARD10 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: CARD10 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for CARD10

Target: CARD10 (Caspase Recruitment Domain Family, Member 10 (CARD10))

Alternative Name: CARD10

Background: CARD10 Antibody: Apoptosis is related to many diseases and development. Cell death signals are transduced by death domain (DD), death effector domain (DED), and caspase recruitment domain (CARD) containing molecules. CARD containing proteins include some caspases, Apaf-1, CARD4, IAPs, RICK, ARC, RAIDD, Bcl-10, and ASC. A novel CARD-containing protein was recently identified and designated CARD10. This protein belongs to the membrane-associated guanylate kinase-like (MAGUK) family of proteins that can function as molecular scaffolds that assist assembly of signal transduction molecules. CARD 10 interacts with Bcl-10, a Bcl protein that promotes apoptosis, caspase-9 maturation and activation of NF-κ,B. CARD 10 and the related protein CARMA1 also associate with NEMO, the regulatory subunit of the Iκ,K complex, demonstrating its importance in the regulation of NF-κ,B transcription factor activation.

Molecular Weight: Predicted: 116 kDa

Observed: 115 kDa

Gene ID: 29775

UniProt: Q9BWT7

Pathways: S100 Proteins